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A target-induced and equipment-free biosensor for amplified visual detection of pesticide acetamiprid with high sensitivity and selectivity
Lingwen Zeng;  Danhua Zhou;  Jinghua Wu;  Chengshuai Liu;  Junhua Chen
2019
Source PublicationAnalytical Methods
Volume11Issue:9Pages:1168-1173
Abstract

We have developed an equipment-free biosensor for visual pesticide residue (acetamiprid) detection with high sensitivity and selectivity. The target acetamiprid associates with an aptamer sequence and releases trigger DNA to initiate the cyclic signal amplification process. An ingeniously designed hairpin DNA probe was employed as the sensing element, which contains the same sequence as the trigger DNA in the stem part of the hairpin and a G-rich sequence in the loop part of the hairpin. An exonuclease III (Exo III)-mediated target recycling strategy was utilized to achieve quadratic amplification. This two-step cyclic signal amplification results in the generation of numerous G-quadruplex DNAzymes that can catalyze the H2O2-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a colored signal readout, thus providing the amplified colorimetric detection of the target. This assay is ultrasensitive, enabling the visual detection of acetamiprid at concentrations as low as 10 pM without instrumentation. Our proposed sensing system also displays high selectivity and can be applied to determine acetamiprid residues in food samples. Moreover, the assay does not involve any chemical modification of DNA, which is simple and low-cost. This sensing platform provides a promising approach for on-site detection of target molecules in resource-constrained regions because of the easy and straightforward readout of results without the use of sophisticated apparatus.

Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.gyig.ac.cn/handle/42920512-1/10896
Collection环境地球化学国家重点实验室
Affiliation1.School of Food Science and Engineering, Foshan University, Foshan 528000, China
2.Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangzhou 510650, China
3.State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China
Recommended Citation
GB/T 7714
Lingwen Zeng;Danhua Zhou;Jinghua Wu;Chengshuai Liu;Junhua Chen. A target-induced and equipment-free biosensor for amplified visual detection of pesticide acetamiprid with high sensitivity and selectivity[J]. Analytical Methods,2019,11(9):1168-1173.
APA Lingwen Zeng;Danhua Zhou;Jinghua Wu;Chengshuai Liu;Junhua Chen.(2019).A target-induced and equipment-free biosensor for amplified visual detection of pesticide acetamiprid with high sensitivity and selectivity.Analytical Methods,11(9),1168-1173.
MLA Lingwen Zeng;Danhua Zhou;Jinghua Wu;Chengshuai Liu;Junhua Chen."A target-induced and equipment-free biosensor for amplified visual detection of pesticide acetamiprid with high sensitivity and selectivity".Analytical Methods 11.9(2019):1168-1173.
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