Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02

	Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02
	GYIG-IR管理员
	2011
发表期刊	African Journal of Biotechnology》 , ,
卷号	10 期号:42 页码:8403-8413
摘要	The objective of this study was to optimize the submerged cultured conditions of carbonic anhydrase (CA) production from the strain of Bacillus mucilaginosus K02 by mono-factor tests with blank control and orthogonal design methodology. When the bacteria was cultured under the condition of monofactor tests with blank control and orthogonal designs, and conventional mono-factor test and orthogonal designs, respectively, the CA productions increased from 5.32 µU/L under the basic medium to 34.53 and 26.12 µU/L. The kinetic analysis of 5 L-fermentor showed that the specific production rate of CA in the optimized medium was almost equaled to the one obtained in the basic and optimized medium with conventional methods. The result demonstrated that CA synthesis was positively correlated with cell growth, and the mono-factor tests with blank control applied in the optimizing composition of the media could achieve better results in contrast to the conventional method.
关键词	Bacillus Mucilaginosus Carbonic Anhydrase Specific Production Rate Kinetic Analysis Orthogonal
学科领域	环境地球化学
语种	英语
文献类型	期刊论文
条目标识符	http://ir.gyig.ac.cn/handle/352002/6970
专题	环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文
推荐引用方式 GB/T 7714	GYIG-IR管理员. Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02[J]. African Journal of Biotechnology》 , ,,2011,10(42):8403-8413.
APA	GYIG-IR管理员.(2011).Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02.African Journal of Biotechnology》 , ,,10(42),8403-8413.
MLA	GYIG-IR管理员."Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02".African Journal of Biotechnology》 , , 10.42(2011):8403-8413.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Optimization of nutr（155KB）			开放获取	使用许可	浏览下载