| Reduction-sensitive micelles with sheddable PEG shells self-assembled from a Y-shaped amphiphilic polymer for intracellular doxorubicine release | |
| Can Cui; Ping Yu; Ming Wu; Yang Zhang; Lei Liu; Bo Wu; Cai-Xia Wang; Ren-Xi Zhuo; Shi-Wen Huang | |
| 2015 | |
| Source Publication | Colloids and Surfaces B: Biointerfaces
 |
| Volume | 129Issue:1Pages:137-145 |
| Abstract | A new type of shell-sheddable micelles with disulfide linkages between the hydrophobic polyester core and hydrophilic poly(ethylene glycol) (PEG) shell was developed based on Y-shaped amphiphilic polymers mPEG-S-S-(PCL)(2). The micelles were then used for the glutathione-mediated intracellular delivery of the anticancer drug doxorubicin (DOX) into tumor cells. The polymer could self-assemble into micelles with an average diameter of 135 nm in aqueous solution and load DOX at a total content of 3.6%. The hydrophilic PEG shell of these micelles could be shed in the presence of reducing agent dithiothreitol (DTT), which resulted in size change of the micelles. In vitro release studies revealed that DOX-loaded mPEG-S-S-(PCL)(2) micelles exhibited faster DOX release in the presence of DTT. MTT assay demonstrated that DOX-loaded mPEG-S-S-(PCL)(2) micelles showed higher cytotoxicity against 10 mM of glutathione monoester (GSH-OEt) pretreated HeLa cells than that of the non-pretreated ones. Confocal laser scanning microscopy and flow cytometry analyses indicated that DOX-loaded mPEG-S-S-(PCL)(2) micelles were efficiently internalized into HeLa cells and exhibited faster DOX release in GSH-OEt-pretreated cells than in cells with no pretreatment. Endocytosis inhibition results proved that mPEG-S-S-(PCL)(2) micelles entered the cells mainly through the clathrin-mediated endocytosis pathway, and caveolae-mediated endocytosis was involved to a small extent. These results indicate the great potential of the proposed Y-shaped reduction-sensitive polymer for application in effective intracellular anticancer drug delivery. |
| Subject Area | 环境地球化学 |
| Indexed By | SCI |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://ir.gyig.ac.cn/handle/352002/6030 |
| Collection | 环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文 |
| Recommended Citation GB/T 7714 | Can Cui,Ping Yu,Ming Wu,et al. Reduction-sensitive micelles with sheddable PEG shells self-assembled from a Y-shaped amphiphilic polymer for intracellular doxorubicine release[J]. Colloids and Surfaces B: Biointerfaces,2015,129(1):137-145. |
| APA | Can Cui.,Ping Yu.,Ming Wu.,Yang Zhang.,Lei Liu.,...&Shi-Wen Huang.(2015).Reduction-sensitive micelles with sheddable PEG shells self-assembled from a Y-shaped amphiphilic polymer for intracellular doxorubicine release.Colloids and Surfaces B: Biointerfaces,129(1),137-145. |
| MLA | Can Cui,et al."Reduction-sensitive micelles with sheddable PEG shells self-assembled from a Y-shaped amphiphilic polymer for intracellular doxorubicine release".Colloids and Surfaces B: Biointerfaces 129.1(2015):137-145. |
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