Cloning and Sequence Analysis of the Gene Encoding Endopolygalacturonases in the Cotton Black Spot Disease Fungus Alternaria macrospora

	Cloning and Sequence Analysis of the Gene Encoding Endopolygalacturonases in the Cotton Black Spot Disease Fungus Alternaria macrospora
	Wu, Yanyou; Huang Huakun
	2013
Source Publication	JOURNAL OF PURE AND APPLIED MICROBIOLOGY
Volume	7 Issue:SI Pages:13-18
Abstract	The fungus Alternaria macrospora is the causal pathogen of cotton black spot disease in cotton seedlings from the cotyledon period to the seedling stages, causing black spots on leaves and their damping off. Endopolygalacturonases (endoPGs) play major roles in pathogen penetration into plants at the initial phase of invasion. In this phase, the pectic polysaccharides in the plant primary cell walls used as potential barriers against pathogens are digested. The present study aimed to clone and characterize the endoPG-encoding gene ampg-1 in Alternaria macrospora. A 1241 bp open reading frame of ampg-1 was cloned. The nucleotide and its deduced peptide sequence alignment showed that it shared quite high identities with two other Alternaria fungi, i.e. Alternaria alternata and Alternaria citri. The analysis of the predicted amino acid sequence of ampg-1 revealed that the endopolygalacturonase in Alternaria macrospora had four PbH1 domains, which was less than those of five other species. Phytopathogenesis was implicated in the quantity of the PbH1 domains. Therefore, the cotton black spot disease prolonged the latency.
Subject Area	环境地球化学
Indexed By	SCI
Language	英语
Document Type	期刊论文
Identifier	http://ir.gyig.ac.cn/handle/352002/5047
Collection	环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文环境地球化学国家重点实验室
Recommended Citation GB/T 7714	Wu, Yanyou,Huang Huakun. Cloning and Sequence Analysis of the Gene Encoding Endopolygalacturonases in the Cotton Black Spot Disease Fungus Alternaria macrospora[J]. JOURNAL OF PURE AND APPLIED MICROBIOLOGY,2013,7(SI):13-18.
APA	Wu, Yanyou,&Huang Huakun.(2013).Cloning and Sequence Analysis of the Gene Encoding Endopolygalacturonases in the Cotton Black Spot Disease Fungus Alternaria macrospora.JOURNAL OF PURE AND APPLIED MICROBIOLOGY,7(SI),13-18.
MLA	Wu, Yanyou,et al."Cloning and Sequence Analysis of the Gene Encoding Endopolygalacturonases in the Cotton Black Spot Disease Fungus Alternaria macrospora".JOURNAL OF PURE AND APPLIED MICROBIOLOGY 7.SI(2013):13-18.

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