Microbial Diversity and Community Structure on Corroding Concretes

	Microbial Diversity and Community Structure on Corroding Concretes
	Hui Li; Dianfeng Liu; Bin Lian; Yuan Sheng; Hailiang Dong
通讯作者	Bin Lian
	2012
发表期刊	Geomicrobiology Journal
卷号	29 期号:5 页码:450-458
摘要	The objective of this study was to analyze bacterial diversity in two different concrete samples to understand the dominant types of bacteria that may contribute to concrete corrosion. Two concrete samples, HN-1 from the sunny side and HN-2 from dark and damp side, were collected from Zijin Mountain in Nanjing and genomic DNA was extracted. The partial bacterial 16S rRNA gene fragment was PCR amplified and two clone libraries were constructed. Amplified ribosomal DNA restriction analysis (ARDRA) was performed by digestion of the 16S rRNA gene and each unique restriction fragment polymorphism pattern was designated as an operational taxonomic unit (OTU). Phylogenetic trees of bacterial 16S rDNA nucleotide sequences were constructed. Sample HN-1 and HN-2 contained 21 OTUs and 26 OTUs, respectively. Proteobacteria and Planctomycetes were the predominant bacteria in both samples, and they are distributed among Herbaspirillum, Archangium, Phyllobacteriaceae and Planctomycetaceae. Cyanobacteria and Rubrobacter sp. are dominant in HN-1; while Acidobacteriaceae, Adhaeribacter sp. and Nitrospira sp. are predominant in HN-2. This distribution pattern was consistent with local environmental conditions of these two samples. The inferred physiological characteristics of these bacteria, based on relatedness of the DNA clone sequences to cultivated species, revealed different mechanisms of concrete corrosion depending on the local environmental conditions.
关键词	16s Rrna Gene Amplified Ribosomal Dna Restriction Analysis (Ardra) Concrete Diversity Of Bacterial Communities Phylogenetic Analysis
学科领域	环境地球化学
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.gyig.ac.cn/handle/352002/4855
专题	环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文环境地球化学国家重点实验室
通讯作者	Bin Lian
推荐引用方式 GB/T 7714	Hui Li,Dianfeng Liu,Bin Lian,et al. Microbial Diversity and Community Structure on Corroding Concretes[J]. Geomicrobiology Journal,2012,29(5):450-458.
APA	Hui Li,Dianfeng Liu,Bin Lian,Yuan Sheng,&Hailiang Dong.(2012).Microbial Diversity and Community Structure on Corroding Concretes.Geomicrobiology Journal,29(5),450-458.
MLA	Hui Li,et al."Microbial Diversity and Community Structure on Corroding Concretes".Geomicrobiology Journal 29.5(2012):450-458.

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