| Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(II) | |
| Daoyong Zhang; Duu-Jong Lee; Xiangliang Pan | |
| Corresponding Author | Duu-Jong Lee |
| 2012 | |
| Source Publication | Journal of the Taiwan Institute of Chemical Engineers
 |
| Volume | 43Issue:3Pages:450-454 |
| Abstract | The Cu(II) binding properties of loosely bound extracellular polymeric substances (LBEPSs) and tightly bound EPSs (TBEPSs) extracted from biofilm samples at two apparent molecular weight (AMW) ranges, >14 kDa and 1-14 kDa, were investigated using three-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy. The protein-like and aromatic protein fluorescence peaks were identified in EEM fluorescence spectra as peaks A and B, respectively. The intensities of peaks A and B were generally quenched when Cu(II) was bound with LBEPSs or TBEPSs with AMWs > 14 kDa at various pH levels. Conversely, for 1-14 kDa EPSs, fluorescence intensities of peaks A and B were not quenched when Cu(II) was bound with LBEPSs at pH 4 or with both LBEPSs and TBEPSs at pH 8. The Stern-Volmer constant (log K-sv) for the Cu(II)-LBEPSs and Cu(II)-TBEPSs binding processes were 2.38-4.37. The capability of EPSs to bind with Cu(II) increased as pH increased. At pH > 4, the protein-like substances and aromatic proteins in TBEPSs had greater Cu(II) binding capability than LBEPSs. Additionally, the EPSs with AMWs > 14 kDa had stronger binding capability with Cu(II) than EPSs with AMWs of 1-14 kDa. The difference in Cu binding behavior of LBEPS and TBEPS significantly affect the mobility, bioavailability, and toxicity of Cu in aquatic environments. |
| Keyword | Extracellular Polymeric Substances Copper Binding Fluorescence Quenching Titration |
| Subject Area | 环境地球化学 |
| Indexed By | SCI |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://ir.gyig.ac.cn/handle/352002/4848 |
| Collection | 环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文 |
| Corresponding Author | Duu-Jong Lee |
| Recommended Citation GB/T 7714 | Daoyong Zhang,Duu-Jong Lee,Xiangliang Pan. Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(II)[J]. Journal of the Taiwan Institute of Chemical Engineers,2012,43(3):450-454. |
| APA | Daoyong Zhang,Duu-Jong Lee,&Xiangliang Pan.(2012).Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(II).Journal of the Taiwan Institute of Chemical Engineers,43(3),450-454. |
| MLA | Daoyong Zhang,et al."Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(II)".Journal of the Taiwan Institute of Chemical Engineers 43.3(2012):450-454. |
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| Fluorescent quenchin（802KB） | 开放获取 | License | View Application Full Text | |||
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