Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(II)

	Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(II)
	Daoyong Zhang; Duu-Jong Lee; Xiangliang Pan
通讯作者	Duu-Jong Lee
	2012
发表期刊	Journal of the Taiwan Institute of Chemical Engineers
卷号	43 期号:3 页码:450-454
摘要	The Cu(II) binding properties of loosely bound extracellular polymeric substances (LBEPSs) and tightly bound EPSs (TBEPSs) extracted from biofilm samples at two apparent molecular weight (AMW) ranges, >14 kDa and 1-14 kDa, were investigated using three-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy. The protein-like and aromatic protein fluorescence peaks were identified in EEM fluorescence spectra as peaks A and B, respectively. The intensities of peaks A and B were generally quenched when Cu(II) was bound with LBEPSs or TBEPSs with AMWs > 14 kDa at various pH levels. Conversely, for 1-14 kDa EPSs, fluorescence intensities of peaks A and B were not quenched when Cu(II) was bound with LBEPSs at pH 4 or with both LBEPSs and TBEPSs at pH 8. The Stern-Volmer constant (log K-sv) for the Cu(II)-LBEPSs and Cu(II)-TBEPSs binding processes were 2.38-4.37. The capability of EPSs to bind with Cu(II) increased as pH increased. At pH > 4, the protein-like substances and aromatic proteins in TBEPSs had greater Cu(II) binding capability than LBEPSs. Additionally, the EPSs with AMWs > 14 kDa had stronger binding capability with Cu(II) than EPSs with AMWs of 1-14 kDa. The difference in Cu binding behavior of LBEPS and TBEPS significantly affect the mobility, bioavailability, and toxicity of Cu in aquatic environments.
关键词	Extracellular Polymeric Substances Copper Binding Fluorescence Quenching Titration
学科领域	环境地球化学
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.gyig.ac.cn/handle/352002/4848
专题	环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文
通讯作者	Duu-Jong Lee
推荐引用方式 GB/T 7714	Daoyong Zhang,Duu-Jong Lee,Xiangliang Pan. Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(II)[J]. Journal of the Taiwan Institute of Chemical Engineers,2012,43(3):450-454.
APA	Daoyong Zhang,Duu-Jong Lee,&Xiangliang Pan.(2012).Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(II).Journal of the Taiwan Institute of Chemical Engineers,43(3),450-454.
MLA	Daoyong Zhang,et al."Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(II)".Journal of the Taiwan Institute of Chemical Engineers 43.3(2012):450-454.

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